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Merck

SAB4200537

Sigma-Aldrich

Anti-Claudin-5 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(e):

Anti-AWAL, Anti-BEC1, Anti-CLDN5, Anti-CPETRL1, Anti-TMVCF

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41
Konjugat:
unconjugated
application:
IHC
WB
Klon:
polyclonal
Speziesreaktivität:
human
citations:
4
Methode(n):
immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human heart
western blot: 1-2 μg/mL using extracts of OVCAR-3 cells

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~23 kDa

Speziesreaktivität

human

Konzentration

~1.0 mg/mL

Methode(n)

immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human heart
western blot: 1-2 μg/mL using extracts of OVCAR-3 cells

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CLDN5(7122)

Allgemeine Beschreibung

Claudin-5 is a member of claudin family. It is also known as CLDN5, beclin 1, TMVCF and CPETRL1 and is highly expressed in endothelial cells (ECs), including the blood-brain barrier.
Claudin-5 is encoded by the gene mapped to human chromosome 22q11.21.

Spezifität

Anti-Claudin-5specifically recognizes human Claudin-5.

Immunogen

synthetic peptide corresponding to an internal sequence of human claudin-5, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in mouse and rat claudin-5.

Anwendung

Anti-Claudin-5 antibody produced in rabbit has been used in immunofluorescence staining.
Anti-Claudin-5 antibody produced in rabbit may be used in several immunochemical techniques including immunoblotting (~23 kDa) and immunohistochemistry.

Biochem./physiol. Wirkung

Claudin-5 gene is a downstream target of ETS-related gene (ERG), a transcription factor that regulates endothelial cell-restricted genes and is markedly repressed in response to inflammatory stimuli. Knockout of claudin-5 in mice is lethal, resulting in disruption of in the blood-brain barrier. Claudin-5 has been shown to be overexpressed in various tumors including lung adenocarcinomas but undetectable in squamous cells cell carcinomas.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

ETS-related gene (ERG) controls endothelial cell permeability via transcriptional regulation of the claudin 5 (CLDN5) gene
Yuan L, et al.
Test, 287(9), 6582-6591 (2012)
Marta Justyna Kozieł et al.
Cancers, 12(3) (2020-03-22)
Claudins are major integral proteins of tight junctions (TJs), the apical cell-cell adhesions that enable maintaining polarity of epithelial cells, their differentiation, and cell signaling. A number of studies have indicated that claudins might play a crucial role in both
Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas
Paschoud S, et al.
Modern Pathology, 20(9), 947-947 (2007)
Janyerkye Tulyeu et al.
Microorganisms, 7(10) (2019-10-19)
Increased intestinal permeability is thought to underlie the pathogenesis of food allergy. We explore the mechanism responsible for changes in the morphology and function of the intestinal barrier using a rat model of food allergy, focusing on the contribution of

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