S4047

Anti-S6 Kinase antibody produced in rabbit

whole antiserum

• Synonym(e): Anti-p70^S6k
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD00676463
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, WB
• Citations: 9

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 70 kDa
• contains: 15 mM sodium azide
• species reactivity: rat, mouse, human
• technique(s): microarray: suitable, western blot: 1:10,000 using mouse NIH 3T3 fibroblast cell extract
• UniProt accession no.: P23443
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RPS6KB1(6198) ; mouse ... Rps6kb1(72508) ; rat ... Rps6kb1(83840)

Beschreibung

General description

S6 kinases are Ser/Thr protein kinases that are capable of phosphorylating the 40S ribosomal protein S6 in response to mitogen stimulation. S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-S6 Kinase reacts specifically with S6 kinase (70 kDa) in human, rat and mouse.

Immunogen

synthetic peptide corresponding to amino acids 6-23 of human S6 kinase.

Application

Anti- S6 kinase antibody may be used for detection by immunoblotting at a working dilution of 1:10,000 using mouse NIH 3T3 fibroblast cell extract. Detection by immunoblotting was also done in primary cultures of mouse glial cells and in LnCAP, prostate cancer cell line.

Biochem/physiol Actions

The sequence of the immunizing peptide is identical in rat and mouse S6K.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids