R8508

Ribonukleinsäure, Transfer aus Backhefe (S. cerevisiae)

buffered aqueous solution

• Synonym(e): Transfer-RNA, tRNA
• CAS-Nummer: 9014-25-9
• UNSPSC Code: 12352106
• NACRES: NA.55
• MDL number: MFCD00132200
• Form: buffered aqueous solution

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• form: buffered aqueous solution
• concentration: 9-11 mg/mL
• foreign activity: DNase, Nickase, none detected
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Transfer RNA (tRNA) is isolated from baker′s yeast.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription.[1] It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.[2]

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Preparation Note

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)