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Merck

R8154

Sigma-Aldrich

Monoclonal Anti-RAP1 antibody produced in mouse

~2 mg/mL, clone 4C8/1, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-TERF2-Interacting Protein, Anti-TERF2IP

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Konjugat:
unconjugated
application:
ARR
ELISA (i)
WB
Klon:
4C8/1, monoclonal
Speziesreaktivität:
human
citations:
5
Methode(n):
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract.

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

4C8/1, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~47 kDa

Speziesreaktivität

human

Konzentration

~2 mg/mL

Allgemeine Beschreibung

Monoclonal Anti-RAP1 (mouse IgG2b isotype) is derived from the hybridoma 4C8/1 produced by the fusion of mouse myeloma cells (Sp2/0 Ag.14 cells) and splenocytes from BALB/c mice immunized with human RAP1. Ras-related protein 1 (RAP1) protein contains 399 amino acids with an acidic C-terminus featuring a 33 amino acid coiled-coil region and a bipartite nuclear localization signal. It is expressed ubiquitously, is located at telomeres and affects telomer length.
Rap1 (repressor/activator protein) is a constituent of shelterin. It consists of an N-terminal BRCT motif, a C-terminal protein interaction domain, and one or two central Myb domains. It is also known as Terf2ip (TERF2 interacting protein). It is located on chromosome 16q23.1.

Immunogen

human RAP1.

Anwendung

Monoclonal Anti-RAP1 antibody produced in mouse may be used in enzyme linked immunosorbent assay (ELISA) and immunoblotting.

Biochem./physiol. Wirkung

Rap1 (repressor/activator protein) helps to guard telomeres, assemble telomerase, and controls telomere length. At telomeres, Rap1 also participates in the suppression of non-homologous end joining (NHEJ). It involves in genomewide transcriptional modulation. Knockdown of Rap1 induces apoptosis in hepatocellular carcinoma cell line HepG2.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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    Downregulation of Rap1 promotes 5-fluorouracil-induced apoptosis in hepatocellular carcinoma cell line HepG2
    Zha Y, et al.
    Oncology Reports, 31(4), 1691-1698 (2014)
    Decoding telomere protein Rap1: Its telomeric and nontelomeric functions and potential implications in diabetic cardiomyopathy
    Cai Y, et al.
    Cell Cycle, 16(19), 1765-1765 (2017)
    Mammalian RAP1 controls telomere function and gene expression through binding to telomeric and extra-telomeric sites
    Martinez P, et al.
    Nature Cell Biology, 12(8), 768?780-768?780 (2010)
    TALEN gene knockouts reveal no requirement for the conserved human shelterin protein Rap1 in telomere protection and length regulation
    Kabir S, et al.
    Cell Reports, 9(4), 1273-1280 (2014)
    Genetic variants in eleven telomere-associated genes and the risk of incident cardio/cerebrovascular disease: The Women's Genome Health Study
    Zee RY, et al.
    Clinica Chimica Acta; International Journal of Clinical Chemistry, 412(1-2), 199-202 (2011)

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