P8398

Phospholipase D aus Kohl

Type IV, lyophilized powder, ≥100 units/mg solid

• Synonym(e): Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
• CAS-Nummer: 9001-87-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-639-8
• MDL number: MFCD00131897
• EG-Nummer: 3.1.4.4 (BRENDA, IUBMB)
• Specific activity: ≥100 units/mg solid

Eigenschaften

• type: Type IV
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥100 units/mg solid
• composition: Protein, ~25%
• storage temp.: −20°C

Beschreibung

General description

Phospholipase D (PLD) from cabbage comprises the C2 domain, PLD superfamily specific conserved regions (I–IV) with HxKx4Dx6GSxN (HKD) motif. Structurally, it is a barrel-shaped monomer bound to calcium ions.

Application

Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.

The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids.

Biochem/physiol Actions

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions.

The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Analysis Note

Protein determined using biuret.

Other Notes

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)