P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Synonym(e): Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• CAS-Nummer: 77107-46-1
• MDL-Nummer: MFCD00131869
• UNSPSC-Code: 12352202
• NACRES: NA.61

Eigenschaften

• Biologische Quelle: bovine heart
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: 2,500-10,000 units/mg protein
• Zusammensetzung: Protein, ≥85% Lowry
• Methode(n): calcium flux assay: suitable
• UniProt-Hinterlegungsnummer: P62157
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... CALM3(520277)

Beschreibung

Anwendung

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Biochem./physiol. Wirkung

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Einheitendefinition

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Physikalische Form

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Angaben zur Herstellung

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)