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Merck

N8786

Sigma-Aldrich

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal

clone 414, purified from hybridoma cell culture

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41
Konjugat:
unconjugated
application:
EM
ICC
IP
WB
Klon:
414, monoclonal
Speziesreaktivität:
mouse, rat, yeast, Xenopus, human
citations:
10
Methode(n):
electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using HeLa total cell extract

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

414, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 62-210 kDa

Speziesreaktivität

mouse, rat, yeast, Xenopus, human

Konzentration

~3 mg/mL

Methode(n)

electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using HeLa total cell extract

Isotyp

IgG1

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.
Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.

Immunogen

rat liver nuclei extract.

Anwendung

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunoelectron microscopy
  • immunofluorescence

Biochem./physiol. Wirkung

Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

EGF signaling and the origin of axial polarity among the insects
Lynch JA, et al.
Current Biology, 20(11), 1042-1047 (2010)
Isolation of the yeast nuclear pore complex.
Rout MP and Blobel G
The Journal of cell biology, 123(4), 771-783 (1993)
The nuclear pore complex ? structure and function at a glance
Greg Kabachinski
Journal of Cell Science (2015)
Lentiviral Vpx induces alteration of mammalian cell nuclear envelope integrity
Singh SP, et al.
Biochemical and biophysical research communications, 511(1) (2019)
Natalia Wesolowska et al.
eLife, 9 (2020-01-29)
The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) that are stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an

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