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Wichtige Dokumente
MAK144
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (orange)
Synonym(e):
Fluorometric ROS Assay Kit
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About This Item
Empfohlene Produkte
Verwendung
sufficient for 200 fluorometric tests (orange)
Anwendung(en)
cosmetics
food and beverages
Nachweisverfahren
fluorometric
Relevante Krankheit(en)
cancer; neurological disorders
Lagertemp.
−20°C
Allgemeine Beschreibung
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.
Anwendung
Fluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV). It is also used to measure the production of intracellular ROS.
Eignung
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.
Prinzip
The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 540/λem = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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