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Merck

M0268

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

MEM

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Qualitätsniveau

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Anwendung

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Leistungsmerkmale und Vorteile

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Menge

Formulated to contain 9.5 grams of powder per liter of medium.

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate.

Sonstige Hinweise

Essentially the same as the original formula, except it is provided as a powder.

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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