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L9420

Sigma-Aldrich

Luciferase aus Glühwürmchen

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Synonym(e):

Luciferase aus Photinus pyralis (firefly)

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About This Item

CAS-Nummer:
61970-00-1
EC-Nummer:
1.13.12.7 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131546
UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in E. coli

Qualitätsniveau

200

Assay

≥98% (SDS-PAGE)

Form

buffered aqueous solution

Spezifische Aktivität

≥10×1010 units/mg protein

Mol-Gew.

62 kDa

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Anwendung

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Einheitendefinition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Physikalische Form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

Zielorgane

Kidney

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

231.8 °F

Flammpunkt (°C)

111 °C

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Sigma-Aldrich

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