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Merck

L9143

Sigma-Aldrich

Lipopolysaccharide aus Pseudomonas aeruginosa 10

purified by phenol extraction

Synonym(e):

LPS

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352201
NACRES:
NA.25

Biologische Quelle

Pseudomonas aeruginosa (10)

Qualitätsniveau

Form

lyophilized powder

Aufgereinigt durch

phenol extraction

Verunreinigungen

<3% Protein (Lowry)

Farbe

white to faint yellow

Löslichkeit

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

This product is phenol extracted from Pseudomonas aeruginosa serotype 10.22 The source strain is ATCC 27316.

Anwendung

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Bingji Jin et al.
Experimental animals, 67(3), 337-347 (2018-03-13)
The epithelial sodium channel (ENaC) and mitogen-activated protein kinase (MAPK) pathway have been reported to be associated with the progression of acute lung injury (ALI). Oxymatrine (OMT) alone or combined with other drugs can ameliorate paraquat- or oleic acid-induced lung
Lesley Berghuis et al.
Veterinary research, 45, 105-105 (2014-10-12)
Bovine respiratory disease is a complex of bacterial and viral infections of economic and welfare importance to the beef industry. Although tracheal antimicrobial peptide (TAP) has microbicidal activity against bacterial pathogens causing bovine respiratory disease, risk factors for bovine respiratory
Kettani-Halabi Mohamed et al.
Plant signaling & behavior, 10(3), e1000160-e1000160 (2015-03-12)
Lipopolysaccharides (LPS) are a component of the outer cell surface of almost all Gram-negative bacteria and play an essential role for bacterial growth and survival. Lipopolysaccharides represent typical microbe-associated molecular pattern (MAMP) molecules and have been reported to induce defense-related
Prunelle Waldman et al.
Environmental science & technology, 51(23), 13633-13640 (2017-11-09)
Although the interaction between phages and bacteria has already been well described, it only recently emerged that human viruses also interact with bacteria in the mammalian gut. We studied whether this interaction could occur in tap water and thus confer
Per-Ola Onnervik et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 59(10), 817-825 (2010-04-14)
The aim was to create pathological changes in mice relevant to human smoke exposure that can be used to further understand the mechanisms and pathology of smoke-induced inflammatory disease. Mice were exposed to tobacco smoke or lipopolysaccharide (LPS) to generate

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