Polystyrene beads are available in several formats - unmodified, carboxyl-modified, or amine-modified - as well as various colors and numberous sizes. This particular product is an amine-modified bead containing a blue fluorescent dye. See the image below to review the basic structure. This is a surface modification that allows for the attachment of certain ligands such as polypeptides via the carboxyl-terminus. The dyes are embedded in the polystyrene bead during the manufacturing process. These are not bound to the surface of the bead.
L5530
Latex beads, carboxylate-modified polystyrene, fluorescent orange
aqueous suspension, 0.5 μm mean particle size
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Form
aqueous suspension
Qualitätsniveau
Zusammensetzung
Solids, 2.5%
Mittlere Partikelgröße
0.5 μm
Fluoreszenz
λex ~520 nm; λem ~540 nm
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Anwendung
Carboxylate-modified polystyrene latex beads (fluorescent orange) have been used to block phagocytosis by hemocytes in Drosophila.[1] It has also been used to assess the uptake of latex beads by antigen presenting cells from porcine respiratory system.[2]
Carboxylate-modified polystyrene latex beads have been used to collect morphofunctional data about the immune systems of Carabus lefebvrei, a helicophagous Italian endemic ground beetle often used as an indicator of the habitat quality of the Apennines mountain forests. Carboxylate-modified polystyrene latex beads have also been used to inform comparative studies on immunity defense mechanisms of adults and larvae of the coleopteran Cetonischema aeruginosa and to investigate the phagocytic activity of cultured rat thyroid cells.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Adam Bajgar et al.
PLoS pathogens, 14(4), e1007022-e1007022 (2018-04-28)
Phagocytosis by hemocytes, Drosophila macrophages, is essential for resistance to Streptococcus pneumoniae in adult flies. Activated macrophages require an increased supply of energy and we show here that a systemic metabolic switch, involving the release of glucose from glycogen, is
Bente Winkler et al.
Science advances, 7(44), eabh0050-eabh0050 (2021-10-28)
[Figure: see text].
Yu Long Han et al.
Nature physics, 16(1), 101-108 (2020-09-10)
Sculpting of structure and function of three-dimensional multicellular tissues depend critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events, and their disruption in disease, remain poorly understood. Using a multicellular
P G Giulianini et al.
Tissue & cell, 35(4), 243-251 (2003-08-19)
In the context of comparative studies on immunity defence mechanisms of adults and larvae of the coleopteran Cetonischema aeruginosa (Drury, 1770) the ultrastructure of the circulating hemocytes of the third instar larval stage has been investigated by means of light
K Takazawa et al.
Acta endocrinologica, 117(2), 198-204 (1988-02-01)
We investigated the phagocytic activity of cultured rat thyroid cells (FRTL) by using latex beads. FRTL cells ingested many carboxylate-modified polystyrene latex beads (c-latex beads), which had carboxyl residues on the surface, whereas they ingested few polystyrene latex beads (p-latex
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Does the polystyrene bead have amine groups attached to the bead surface? What is the purpose of this modification? How is the dye bound to these beads.
1 answer-
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Can this product be used in animal rodent model for in vivo imaging using Perkin Elmer IVIS Spectrum platform???
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Product L5530, Latex beads, carboxylate-modified polystyrene, is not tested for fluorescent orange in animal rodent model for in vivo imaging using Perkin Elmer IVIS Spectrum platform. It is encouraged that the end-user determines the suitability of this application.
Please note that the fluorescence is : λex ~520 nm; λem ~540 nm
Helpful?
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What does "λex ~520 nm" mean? In particular, I do not understand the meaning of "~". Is it possible to excite with excitation wavelengths from 430 to 510 nm? 「λex ～520 nm」とはどういう意味ですか？ 特に「～」の意味がわかりません。 430～510nmの励起波長で励起できるのですか？
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Product L5530 is a fluorescent product having λex ~520 nm; λem ~540 nm, where λex ~520 nm means excitation at 520nm and λem ~540 nm means emission at 540nm. The spectra is quite tight and hence it will be important to set the filters accordingly. "~" means "about/around" in this aspect, where if λex is 520 nm, +/- 5 to 10 nm difference in the wavelength should be alright.
Generally, a range of 430-510nm ex/em is not suitable for green fluorochomes. The 430 nm wavelength is too early for excitation and is not recommended for this product.Helpful?
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