L3295

Phospholipase A1 from Aspergillus oryzae

• Synonym(e): PLA1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.1.1.32 (BRENDA, IUBMB)
• MDL number: MFCD00130786
• Specific activity: ≥10 KLU/g
• Recombinant: expressed in Aspergillus oryzae

Eigenschaften

• recombinant: expressed in Aspergillus oryzae
• Quality Segment: 200
• form: liquid
• specific activity: ≥10 KLU/g
• storage temp.: 2-8°C

Beschreibung

General description

Phospholipase A1 (PLA1) catalyzes the hydrolysis of acyl group from position 1 of lecithin to yield lysolecithin. It is expressed in a wide range of organisms such as rat platelets, bovine brain and testis, hornet venom, bonito muscle and fungi. Gene coding for PLA1 consists of four exons and three short introns spanning 1,056bp of genomic DNA. Mature protein contains 269 aminoacids and two possible N-glycosylation sites (Asn27 and Asn55).

Application

Phospholipase A1 from Aspergillus oryzae has been used:

• in the preparation of sn-1 and sn-2 C18:1- lysophosphatidylcholine (LPC) regioisomer standards
• as a catalyst for the synthesis 6-O-glucosyl-poly(3-hydroxyalkanoates) in a micro-aqueous system
• to catalyze the synthesis of methyl butanoate and methyl benzoate flavor esters in continuous flow microreactor
• to hydrolyze 17:0 phosphocholine (PC)

Analysis Note

minimum activity 10 KLU/G liquid

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable