GUSS

β-Glucuronidase Reporter Gene Staining Kit

• Synonym(e): β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
• UNSPSC-Code: 12161503
• NACRES: NA.32

Eigenschaften

• Verwendung: kit sufficient for 100 histochemical assays
• Qualitätsniveau: 200
• Methode(n): microbe id | staining: suitable
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Anwendung

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Leistungsmerkmale und Vorteile

• Histochemical staining of plant tissues expressing the E. coli GUS enzyme
• Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
• GUS does not interfere with plant cell function or viability

Sonstige Hinweise

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Nur Kit-Komponenten

• 2× Fixation Buffer 25 mL
• 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
• Reagent A for gus staining kit 50 mL
• Reagent B for gus staining kit 200 μL
• Reagent C for gus staining kit 200 μL

Sicherheitshinweise

• H-Sätze: H412
• P-Sätze: P273 - P501
• Gefahreneinstufungen: Aquatic Chronic 3
• Lagerklassenschlüssel: 11 - Combustible Solids