GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
Synonym(e):
Golgi Kit, Isolation Kit for Golgi
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About This Item
Empfohlene Produkte
Verwendung
sufficient for 50 g (tissue)
Qualitätsniveau
Methode(n)
fractionation: suitable
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Anwendung
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Hinweis zur Analyse
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDB
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
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