GE28-4017-47
GSTrap™ 4B
Cytiva 28-4017-47, pack of 1 × 5 mL
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)
About This Item
Empfohlene Produkte
ligand
glutathione
Verpackung
pack of 1 × 5 mL
Hersteller/Markenname
Cytiva 28-4017-47
Parameter
42 psi (H2O at 25°C)
Bettgröße
16 mm × 25 mm
Bettvolumen
5 mL
Säule ID
16 mm
Matrix
4% agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning
4-13
working range
4-13
Kapazität
>25 mg binding capacity (horse liver GST/ml medium)
Allgemeine Beschreibung
GSTrap™ 4B columns are prepacked Glutathione Sepharose™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Anwendung
GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Leistungsmerkmale und Vorteile
- High binding capacity.
- Mild elution conditions preserving Protein antigenicity and function.
- Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
- GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.
Lagerung und Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Hinweis zur Analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Sonstige Hinweise
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Rechtliche Hinweise
FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
The Journal of cell biology, 220(9) (2021-06-25)
The Scar/WAVE complex drives actin nucleation during cell migration. Interestingly, the same complex is important in forming membrane ruffles during macropinocytosis, a process mediating nutrient uptake and membrane receptor trafficking. Mammalian CYRI-B is a recently described negative regulator of the
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.