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G4509

Sigma-Aldrich

Glycerokinase aus E. coli

lyophilized powder, 40-100 units/mg protein

Synonym(e):

ATP:glycerol 3-phosphotransferase, Glycerin-Kinase

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About This Item

CAS-Nummer:
9030-66-4
EC-Nummer:
2.7.1.30 (BRENDA, IUBMB)
EG-Nummer:
232-862-0
MDL-Nummer:
MFCD00131205
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

Escherichia coli

Qualitätsniveau

200

Form

lyophilized powder

Spezifische Aktivität

40-100 units/mg protein

Zusammensetzung

Protein, ≥50% biuret

Fremdaktivität

Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%

Lagertemp.

−20°C

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Anwendung

Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis.

Biochem./physiol. Wirkung

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. <<<20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate.<<<22>>>

Einheitendefinition

1 U setzt 1.0 μmol Glycerin und ATP zu L-α-Glycerophosphat und ADP pro Minute um bei pH 9.8 und 25°C in einem gekoppelten System mit PK/LDH.

Physikalische Form

Teilweise gereinigtes lyophilisiertes Pulver, Rest ist vorwiegend Salze und EDTA

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Sigma-Aldrich

L9518

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F Lönnqvist et al.
Arteriosclerosis, thrombosis, and vascular biology, 17(7), 1472-1480 (1997-07-01)
Cardiovascular complications of obesity are more common in men than women. Sex differences in visceral fat lipolysis may be of importance in this respect, since increased release of free fatty acids (FFAs) from visceral fat to the liver by the
V Van Harmelen et al.
The Journal of biological chemistry, 274(26), 18243-18251 (1999-06-22)
The effects of acylation-stimulating protein (ASP) and insulin on free fatty acid (FFA) release from isolated human fat cells and the signal transduction pathways to induce these effects were studied. ASP and insulin inhibited basal and norepinephrine-induced FFA release by
A Girard et al.
The Biochemical journal, 274 ( Pt 3), 819-824 (1991-03-15)
An artificial-membrane-bound glycerokinase chosen as a membrane-bound two-substrate-enzyme model has been used to separate two unequal compartments of a specially designed diffusion cell. An interesting feature is the asymmetry of compartments and the existence of a diffusion layer adjacent to
S Reynisdottir et al.
Diabetologia, 37(4), 428-435 (1994-04-01)
Upper-body obesity is an important risk factor for developing non-insulin dependent diabetes. To investigate the possibility that a lipolysis defect is present in this form of obesity, we examined the adrenergic regulation of lipolysis in abdominal subcutaneous fat cells from
L Hellström et al.
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, 21(4), 314-320 (1997-04-01)
The weight loss achieved during treatment with very-low-calorie diets (VLCD) varies between individuals. The aim of this study was to investigate whether interindividual variations in catecholamine-induced lipolysis are of importance for the rate of weight loss during VLCD. Prospective study.
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