G4040

Anti-Gα _i3 antibody produced in rabbit

whole antiserum, buffered aqueous solution

• Synonym(e): Anti-87U6, Anti-ARCND1, Anti-HG1A
• MDL-Nummer: MFCD00239679
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: whole antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~40 kDa
• Speziesreaktivität: mouse, human, rat, hamster
• Methode(n): immunoprecipitation (IP): suitable; western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
• UniProt-Hinterlegungsnummer: P08754
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GNAI3(2773) ; mouse ... Gnai3(14679) ; rat ... Gnai3(25643)

Beschreibung

Allgemeine Beschreibung

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

Spezifität

Recognizes Gα_i3 and Gα₀ G-proteins.

Immunogen

synthetic peptide corresponding to the C-terminal region of Gα_i3.

Anwendung

Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

Biochem./physiol. Wirkung

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca⁺² mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

Physikalische Form

Solution of whole antiserum diluted in PBS, pH 7.4.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable