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D7304

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

Synonym(e):

Chromogenic substrate, DAB substrate solution

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Über diesen Artikel

NACRES:
NA.46
UNSPSC Code:
12352203
Technischer Dienst
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form

liquid

Quality Level

shipped in

wet ice

storage temp.

2-8°C

General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
  • in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
  • for visualization of sections after immunostaining
  • in immunohistochemistry of mice knee joint sections
  • in the immunolabeling of kisspeptin neurons in the preoptic area of camel
It produces a brown precipitate with peroxidase for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures.

Preparation Note

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Disclaimer

Stable at least one year at 2-8 °C.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • 10X DAB Liquid Chromogen 25 mL

  • ready-to-use buffer/peroxide solution 250 mL

signalword

Danger

target_organs

Eyes,Central nervous system

Lagerklasse

3 - Flammable liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - STOT SE 1



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Verwandter Inhalt

Instructions


S R Pitkin et al.
Behavioural brain research, 119(2), 167-177 (2001-02-13)
The present study aimed to assess the role of advanced age in the development and manifestation of thiamine deficiency using an animal model of Wernicke-Korsakoff syndrome (WKS). Interactions between pyrithiamine-induced thiamine deficiency (PTD) and age were examined relative to working
Adriana Martins Anciães et al.
Clinics (Sao Paulo, Brazil), 66(10), 1797-1803 (2011-10-21)
To verify the accordance of functional and morphometric parameters during the development of emphysema. BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such
Jennifer Walshe et al.
Developmental biology, 264(2), 522-536 (2003-12-04)
Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton



Global Trade Item Number

SKUGTIN
D7304-1SET04061838255235