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CMC0014

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BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(e):

Competent E. coli cells

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About This Item

UNSPSC-Code:
41106202
NACRES:
NA.85

product name

BL21(DE3) Chemically Competent Cells, for protein expression

Biologische Quelle

Escherichia coli

Qualität

for molecular biology

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Anwendung

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Leistungsmerkmale und Vorteile

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Komponenten

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

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Lagerklassenschlüssel

10 - Combustible liquids

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