TM4

88111401, mouse testis, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: TM4, 88111401
• Biologische Quelle: mouse testis
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Transferrin, H-Y Ag, novel retinol-binding protein
• Rezeptoren: Androgen, oestrogen and progesterone receptors
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c testis sertoli cell

Beschreibung der Zelllinie

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Anwendung

Hormone studies

Nährmedium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Sonstige Hinweise

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