3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: 3T3 Swiss Albino, 85022108
• Biologische Quelle: mouse embryo
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 40; hypertriploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

Product has lost contact inhibition

Ursprung der Zelllinie

Mouse Swiss Albino embryo fibroblast

Beschreibung der Zelllinie

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Anwendung

Contact inhibition and virus studies: polyoma and SV40 viruses

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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