While not specifically tested on gingival fibroblasts, this product is tested using mulitple mammalian cell lines. There is no reason to suspect that this product would not be suitable. For frozen cell stocks, the recommended maximum temperature is -70°C. Liquid nitrogen (-80°C) is a typical medium for this product. Please see the link below to review the product datasheet, which includes a brief protocol for freezing and thawing cells using this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/709/138/c6164pis.pdf
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Wichtige Dokumente
C6164
Zellgefriermedium-DMSO 1×
sterile-filtered, suitable for cell culture
Synonym(e):
cryopreservation medium
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352207
NACRES:
NA.75
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Sterilität
sterile-filtered
Qualitätsniveau
Form
liquid
Methode(n)
cell culture | mammalian: suitable
cryopreservation: suitable
Verunreinigungen
endotoxin, tested
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Das Einfrieren von Zellen oder die Kryokonservierung von Zellkulturen ist ein Routineprozess. Er ist dringend erforderlich, um während der Lagerung die Zellintegrität zu bewahren. Zu den häufig verwendeten Kälteschutzmitteln zählen Glycerin, Ethylenglycol und Dimethylsulfoxid (DMSO).[1]
Anwendung
Sonstige Hinweise
Vollständiges gebrauchsfertiges Medium. Proprietäre Formulierung mit Minimum Essential Medium, Dimethylsulfoxid, Kälberserum und fetalem Kälberserum.
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves
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Good day will the cell- freezing medium - DMSO be suitable for freezing of human gingival fibroblsts? can they be stored in -80 freezer or rather in liquid nitrogen with this freezer medium?
1 answer-
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When is the expiry date after bottle has been opened?
1 answer-
Following opening, the stability of the product is contingent upon end user handling. Shelf life will be maintained unless contaminants are introduced or the product is improperly stored or sealed. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?
1 answer-
The DMSO is at a 10% concentration.
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Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?
1 answer-
Product No. C6164 is sterile-filtered. If the sterility of the product has been compromised, it can be re-filtered.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum?
1 answer-
No, the serum in this cell freezing media is not heat-inactivated.
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Is there insulin in Product C6164, Cell Freezing Medium-DMSO?
1 answer-
No, there is no added insulin in this product. There may be traces of insulin from the serum used in this product.
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