A6805

β-N-Acetylglucosaminidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
• CAS-Nummer: 9012-33-3
• EC-Nummer: 3.2.1.52 (BRENDA, IUBMB)
• EG-Nummer: 232-730-2
• MDL-Nummer: MFCD00081402
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 100
• Form: buffered aqueous solution
• Spezifische Aktivität: ≥80 units/mg protein
• Verpackung: vial of ≥1.0 unit
• Fremdaktivität: β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Angaben zum Gen: Streptococcus pneumoniae R6 ... lytB(934406)

Beschreibung

Anwendung

β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.

Biochem./physiol. Wirkung

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.

Specific for GlcNAc; not significantly active with GalNAc

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

Physikalische Form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable