A4335

Monoklonaler Anti-Myosin-Antikörper (skelettal, Fast) in Maus hergestellte Antikörper

clone MY-32, purified from hybridoma cell culture

• Synonym(e): Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00145920
• Conjugate: alkaline phosphatase conjugate
• Clone: MY-32, monoclonal
• Application: IF (d)
• Citations: 24

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MY-32, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
• technique(s): direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections
• isotype: IgG1
• UniProt accession no.: P12882
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MYH1(4619), MYH2(4620) ; mouse ... Myh1(17879), Myh2(17882) ; rat ... Myh1(287408), Myh2(691644)

Beschreibung

General description

Lokalisiert ein Epitop in der schweren Kette des Myosins. Färbt die schnellen (Typ II) und neonatalen Isomyosinmoleküle, die in der Skelettmuskulatur vorkommen, aber nicht das Myosin aus Herzmuskel, glatter Muskulatur oder anderen Geweben als Muskeln in kultivierten Zellen. Reagiert mit humanen Rhabdomyosarkomen.

Monoclonal Anti-Myosin (Skeletal, Fast) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Myosin aus Kaninchenmuskel.

Application

Die Konzentration von Myosin (Fast) in Serumproben von Sportlern mit vorangegangener Verletzung wurde im Western Blot mittels eines monoklonalen Anti-Myosin-Antikörpers von der Maus (skelettal/Fast) als primärer Antikörper in einer Verdünnung von 1:90000 bestimmt.

Monoclonal Anti-Myosin (Skeletal, Fast) Alkaline Phosphatase antibody produced in mouse has been used for the detection and localization of skeletal muscle fast and neonatal myosins using immunohistochemistry. It may be used in muscle fiber typing, in studies of in vivo and in vitro muscle development and in the diagnosis of rhabdomyosarcomas.

Biochem/physiol Actions

Monoclonal Anti-Myosin (Skeletal, Fast) specifically binds to an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers)†. The antibody product is also reactive in humans, bovines, cats, rabbits, mice, guinea pigs and chickens.

Myosin (Skeletal, Fast) is useful not only in fiber typing but also in detection of myogenic tumors. It enables retrospective as well as prospective fiber typing using formalin-fixed, paraffin-embedded material.

Myosins are motor proteins that interact with actin filaments to regulate cell movement. Myosins also modulate cell shape and signaling. Skeletal muscle myosins have three ′fast′ myosin heavy chains isoforms (IIa, IIx, and IIb), a ′slow′ beta-myosin heavy chain isoform and three major myosin light-chain (MLC) isoforms. These myosin isoforms regulate skeletal muscle shortening.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2