Calcein-AM is a fluorescent dye used to stain live bacterial cells for flow cytometry application. Product 17783, Calcein-AM, is suitable for fluorescence and is at least 96.0% HPLC pure. For dyes that can stain bacteria but do not enter bacterial cells, you may find the products SCT126, BioTracker 510 Green C4(FM1-43) Synaptic Dye, and SCT127, BioTracker 640 Red C2(FM4-64) Synaptic Dye may be suitable.
Alle Fotos(1)
Wichtige Dokumente
17783
Calcein-AM
suitable for fluorescence, BioReagent, ≥90% (HPLC)
Synonym(e):
Calcein-acetoxymethylester, Calcein-O,O′-diacetat-tetrakis-(acetoxymethyl)-ester
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
Empirische Formel (Hill-System):
C46H46N2O23
CAS-Nummer:
Molekulargewicht:
994.86
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.32
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Produktlinie
BioReagent
Qualitätsniveau
Assay
≥90% (HPLC)
Fluoreszenz
λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
Eignung
suitable for fluorescence
Lagertemp.
−20°C
SMILES String
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChIKey
XKFSBWQWNMZWFA-UHFFFAOYSA-N
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Allgemeine Beschreibung
Liposome enthalten den selbstlöschenden Fluoreszenzfarbstoff Calcein. Dieser Fluoreszenzfarbstoff hat ein Anregungsmaximum von 495 nm und ein Abstrahlungsmaximum von 515 nm. Eine Konzentration über 70 mM führt zur Selbstlöschung, was häufig als Indikator für undichte Lipidvesikel verwendet wird. Die Leckrate wird auf Basis des Fluoreszenz-Inkrements berechnet, während das eingeschlossene Calcein aus dem liposomalen Kompartement ausläuft. Wird als Indikator für komplexometrische Titrationen von Calciumionen mit EDTA und für die fluorometrische Bestimmung von Calcium verwendet.[1][2]
Anwendung
Calcein-AM wurde in der Durchflusszytometrie verwendet.[3]
Nicht-fluoreszierendes zellpermeables Derivat von Calcein, das nach Hydrolyse fluoreszierend wird. Verwendung als neutrales Substrat für Multidrug Resistance Protein, MRP; Einsatz in der Tumorforschung
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Y S Tsao et al.
Biochemistry, 24(5), 1092-1098 (1985-02-26)
Sendai virus induced liposome leakage has been studied by using liposomes containing a self-quenching fluorescent dye, calcein. The liposomes used in this study were prepared by a freeze and thaw method and were composed of phosphatidylcholine, phosphatidylserine, and phosphatidylethanolamine (1:2.60:1.48
T M Allen et al.
Biochimica et biophysica acta, 597(2), 418-426 (1980-04-10)
Efflux of contents from small unilamellar vesicles of various compositions, conaining a highly quenched fluorescent compound (calcein, 175 mM) was determined as a function of temperature in the presence and absence of human serum. Efflux of calcein from the liposomes
Xiao-Cong Huang et al.
Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)
High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors
Bao-Ling Du et al.
Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)
Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs
Johan van der Stok et al.
Tissue engineering. Part A, 21(9-10), 1495-1506 (2015-01-30)
A promising bone graft substitute is porous titanium. Porous titanium, produced by selective laser melting (SLM), can be made as a completely open porous and load-bearing scaffold that facilitates bone regeneration through osteoconduction. In this study, the bone regenerative capacity
-
Recommend fluorescent dyes for live bacterial cell sorting, especially those validated that the dye enters in bacteria and are not transferred to daughter cells.
1 answer-
Helpful?
-
-
What live cell dye (DNA non-binding) is recommended for staining live cells while ensuring that the cells remain viable after staining, particularly for bacterial cells? Would using DSMO be suitable, or is there an alternative stain recommendation?
1 answer-
Calcein AM may not be soluble in water. DMSO can be used as the solvent and there is no mention of solubility in water or alcohol. The free acid is soluble in water and ethanol. However, only DMSO is listed for the solubility of Calcein AM. Concerns about DMSO affecting bacteria viability may lead to a switch from the hexa ester to the free acid form. Maintaining a 1:500 dilution of DMSO in the staining solution is advisable to avoid potential viability issues.
Helpful?
-
-
How is Calcein-AM, Product 17783, typically used?
1 answer-
Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.
Helpful?
-
-
What are the spectral properties of Calcein-AM, Product 17783?
1 answer-
The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the solubility of Calcein-AM, Product 17783?
1 answer-
Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).
Helpful?
-
-
Do the Scienceware® brand polypropylene products contain any leachable material?
1 answer-
Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.
Helpful?
-
-
What is the appearance of Calcein-AM, Product No. 17783?
1 answer-
Product No. 17783 can be a film, oil, paste or solid.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
