T17917

Tricin

≥99% (titration), reagent grade, crystalline

• Synonym(e): N-[Tris-(hydroxymethyl)-methyl]-glycin
• Lineare Formel: (HOCH₂)₃CNHCH₂CO₂H
• CAS-Nummer: 5704-04-1
• Molekulargewicht: 179.17
• Beilstein: 1937804
• MDL-Nummer: MFCD00004277
• UNSPSC-Code: 12161700
• NACRES: NA.25

Eigenschaften

• Biologische Quelle: synthetic
• Qualitätsniveau: 200
• Qualität: reagent grade
• Assay: ≥99% (titration)
• Form: crystalline
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: <0.5% water
• Farbe: white
• Nützlicher pH-Bereich: 7.4-8.8
• pKa (25 °C): 8.1
• mp (Schmelzpunkt): 187 °C
• Löslichkeit: water: 0.1 M, clear, colorless
• Kationenspuren: Pb: ≤5 ppm
• Lagertemp.: room temp
• SMILES String: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChIKey: SEQKRHFRPICQDD-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Anwendung

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

Pufferkomponente für die Trennung von Peptiden mit geringer Molekülmasse.

Leistungsmerkmale und Vorteile

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Sonstige Hinweise

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Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable