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11427598910

Roche

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

Synonym(e):

Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate

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About This Item

Empirische Formel (Hill-System):
C32H52N7O17P3S
CAS-Nummer:
188755-42-2
Molekulargewicht:
931.78
UNSPSC-Code:
41116100

Qualitätsniveau

100

Assay

≥98% (HPLC)

Form

solution

Mol-Gew.

955.5 (biotin-16-ddUTP-Li4)

Verpackung

pkg of 25 μL (25 nmol; 1mM)

Hersteller/Markenname

Roche

Konzentration

1 mM

Methode(n)

hybridization: suitable

Lagertemp.

−20°C

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Allgemeine Beschreibung

Biotin-16-ddUTP, tetralithium salt; 1mM

Anwendung

  • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
  • DNA polymerase I (Holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.
  • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Leistungsmerkmale und Vorteile

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Qualität

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

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