NA46

Anti-p300 Mouse mAb (NM-11)

liquid, clone NM-11, Calbiochem^®

• Synonym(e): Anti-p300 antibody
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: NM-11, monoclonal
• Form: liquid
• Enthält nicht: preservative
• Speziesreaktivität: human, rat
• Darf nicht reagieren mit: mouse
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EP300(2033)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.

Recognizes the p300 protein in A431, HeLa, and ML-1 cells.

This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Immunogen

Epitope: within amino acids 2071-2091 near the CBP C-terminus

Human

recombinant, human p300 protein

Anwendung

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
A431, HeLa, or ML-1 cells

Sonstige Hinweise

Antibody should be titrated for optimal results in individual systems.

Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3