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MABS1994M

Sigma-Aldrich

Anti-MT-ND4 Antibody, clone 9E4-2D8

clone 9E4-2D8, from mouse

Synonym(e):

NADH-ubiquinone oxidoreductase chain 4, EC: 1.6.5.3, NADH dehydrogenase subunit 4

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

9E4-2D8, monoclonal

Speziesreaktivität

human

Verpackung

antibody small pack of 25 μg

Methode(n)

western blot: suitable

Isotyp

IgG2aκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MT-ND4(4538)

Allgemeine Beschreibung

NADH-ubiquinone oxidoreductase chain 4 (UniProt: P03905; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 4, MT-ND4) is encoded by the MT-ND4 (also known as MTND4, NADH4, ND4) gene (Gene ID: 4538) in human. MT-ND4 is an inner mitochondrial membrane protein that belongs to the complex I subunit 4 family. It is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). The complex couples the oxidation of NADH and the reduction of ubiquinone (Coenzyme Q10), to the generation of a proton gradient, which is then used for ATP synthesis. The complex can be dissociated into two main sub-complexes, corresponding to the "ankle" of the boot, and the "foot" of the boot. The ankle is thought to protrude from the membrane so as to be predominantly in the aqueous phase on the matrix side. It contains the binding site for NAD(H), and the input electron transfer chain. The foot (hydrophobic) is membrane bound and contains a catalytic site at which ubiquinone is reduced. Defects in MT-ND4 gene are known to cause Leber hereditary optic neuropathy (LHON) that is characterized by acute or subacute loss of central vision due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Other diseases associated with MT-ND4 gene mutations are age-related macular degeneration, mesial temporal lobe epilepsy, and cystic fibrosis.

Spezifität

Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.

Immunogen

KLH-conjugated linear peptide from the C-terminal region.

Anwendung

Anti-MT-ND4, clone 9E4-2D8, Cat. No. MABS1994,is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 4 and has been tested for use in Western Blotting.
Western Blotting Analysis: 4 µg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA).

Qualität

Evaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Western Blotting Analysis: 1 µg/mL of this antibody detected MT-ND4 in Mitochondria from human neonatal dermal fibroblasts and did not detect it in mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Zielbeschreibung

~37 kDa observed; 51.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Li-Sheng Zhang et al.
Nature cell biology, 23(7), 684-691 (2021-07-14)
Members of the mammalian AlkB family are known to mediate nucleic acid demethylation1,2. ALKBH7, a mammalian AlkB homologue, localizes in mitochondria and affects metabolism3, but its function and mechanism of action are unknown. Here we report an approach to site-specifically

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