MABS197

Anti-PTP1B Antibody, clone FG6-1G

clone FG6-1G, from mouse

• Synonym(e): Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: FG6-1G, monoclonal
• Speziesreaktivität: human
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_002818
• UniProt-Hinterlegungsnummer: P18031
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PTPN1(5770)

Beschreibung

Allgemeine Beschreibung

PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

Spezifität

This antibody recognizes the catalytic domain of PTP1B.

Immunogen

Epitope: Catalytic domain

Recombinant protein corresponding to the catalytic domain of human PTP1B.

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Cytoskelettale Signalübertragung

Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Western Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).

Qualität

Evaluated by Western Blot in TF-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.

Zielbeschreibung

~50 kDa observed

Verlinkung

Replaces: 04-1140

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
TF-1 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable