MABN238

Anti-Glucagon Antibody, clone 13D11.33

clone 13D11.33, from mouse

• Synonym(e): 4030-01F, 4031-01F, AB932
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 13D11.33, monoclonal
• Speziesreaktivität: human, rat
• Methode(n): ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); multiplexing: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_002045
• UniProt-Hinterlegungsnummer: P01275
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... GCG(2641)

Beschreibung

Allgemeine Beschreibung

Glucagon is a hormone that is proteolytically cleaved into at least eight different functional peptides. It is produced by pancreatic cells and it has binding sites in the brain, intestines, and liver, among other tissues. Glucagon is secreted in response to hypoglycemia. Through the combined activation of Gsα- and Gq-coupled transmembrane receptors and their downstream signaling pathways, glucagon stimulates the release of glucose from the liver into the bloodstream. One important mechanism underlying this process is the activation of protein kinase A (PKA). PKA signals result in increased transcription of phosphoenolpyruvate carboxykinase, which encodes the rate-limiting enzyme in the production of glucose from noncarbohydrate substrates. PKA signals may also increase the breakdown of glycogen, and suppress glycolysis. Glucagon may also engage in calcium-dependent signaling via PLC. The importance of glucagon in regulating glucose homeostasis, in conjunction with insulin, is underscored by findings that glucagon and glucagon receptor signaling are disrupted in type II diabetes.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to human Glucagon.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Sensorik & Peripheres Nervensystem (PNS)

Detect Glucagon using this Anti-Glucagon Antibody, clone 13D11.33 validated for use in IHC(P), Immunofluorescence, ELISA, Multiplexing.

Immunohistochemistry Analysis: A 1:8,000 dilution from a representative lot detected Glucagon in normal rat pancreas tissue (For enhanced staining results, it is recommended to use a 10,000 dilution of this antibody.).

ELISA Analysis: A representative lot detected Glucagon in ELISA.

Luminex^® Analysis: A representative lot detected Glucagon in Luminex Assay.

Qualität

Evaluated by Immunofluorescence in normal rat pancreas tissue.

Immunofluorescence Analysis: A 1:8,000 dilution of this antibody detected Glucagon in normal rat pancreas tissue.

Zielbeschreibung

21 kDa calculated

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Normal rat pancreas tissue

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

Luminex is a registered trademark of Luminex Corp

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable