MABF148C

Anti-Ly-6G (mouse), redFluor^® 710, clone RB6-8C5 Antibody

clone RB6-8C5, from rat, redFluor^® 710

• Synonym(e): Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: redFluor^® 710
• Clone: RB6-8C5, monoclonal
• Application: FACS
• Citations: -

Eigenschaften

• biological source: rat
• Quality Segment: 100
• conjugate: redFluor^® 710
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RB6-8C5, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: P35461
• target post-translational modification: unmodified
• Gene Information: mouse ... Ly6G(546644)

Beschreibung

General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Application

Anti-Ly-6G (mouse), redFluor^® 710, clone RB6-8C5 Antibody is an antibody against Ly-6G for use in Flow Cytometry.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.015 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

redFluor is a registered trademark of Tonbo Biotechnologies

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable