MAB3785

Anti-APC Antibody, NT, clone Ali 12.28

clone Ali 12.28, Chemicon^®, from mouse

• Synonym(e): Adenomatous Polyposis Coli Protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: Ali 12.28, monoclonal
• Speziesreaktivität: human
• Darf nicht reagieren mit: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_000038.3
• UniProt-Hinterlegungsnummer: P25054
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... APC(324) ; mouse ... Apc(11789)

Beschreibung

Allgemeine Beschreibung

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Spezifität

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Immunogen

Epitope: N-terminus

N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Verlinkung

Replaces: AB1268

Physikalische Form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Hinweis zur Analyse

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable