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MAB1222A

Sigma-Aldrich

Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase

clone G3, Chemicon®, from mouse

Synonym(e):

Anti-TPS1, Anti-TPSB1, Anti-TPSB2, Anti-Tryptase-2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Konjugat

alkaline phosphatase conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

G3, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Chemicon®

Methode(n)

immunohistochemistry: suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

NM_003294.3

UniProt-Hinterlegungsnummer

Q15661

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... TPSAB1(7177)

Spezifität

Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

Anwendung

Research Category
Entzündung & Immunologie
Research Sub Category
Entzündungs- & Autoimmunmechanismen
Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH.
Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky′s reagent [Lab. Invest. (1988). 58:682-691].

Optimal working dilutions must be determined by end user.

Physikalische Form

In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0.

Lagerung und Haltbarkeit

Maintain at +2-8°C in undiluted aliquots for up to 6 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

David Lamb et al.
Respiratory research, 22(1), 158-158 (2021-05-24)
RORγt is a transcription factor that enables elaboration of Th17-associated cytokines (including IL-17 and IL-22) and is proposed as a pharmacological target for severe asthma. IL-17 immunohistochemistry was performed in severe asthma bronchial biopsies (specificity confirmed with in situ hybridization).
Premkumar Siddhuraj et al.
Frontiers in immunology, 13, 924244-924244 (2022-08-20)
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics and spatial distribution of mast cell CPA3 expression in lung diseases remain unknown. Using a histology-based approach for quantitative spatial
Premkumar Siddhuraj et al.
Cells, 10(2) (2021-02-07)
The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on

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