AB9876
Anti-Stargazin Antibody
Chemicon®, from rabbit
Synonym(e):
Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Speziesreaktivität
rat, mouse
Hersteller/Markenname
Chemicon®
Methode(n)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
mouse ... Cacng2(12300)
rat ... Cacng2(84347)
Spezifität
Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum.
Immunogen
GST fusion protein from rat stargazin, cytoplasmic domain.
Anwendung
Research Category
Neurowissenschaft
Neurowissenschaft
Research Sub Category
Synapsen & Synapsenbiologie
Synapsen & Synapsenbiologie
This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin.
Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Physikalische Form
ImmunoAffinity Purified
Format: Purified
Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4.
Lagerung und Haltbarkeit
Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.
Ma, TM; Paul, BD; Fu, C; Hu, S; Zhu, H; Blackshaw, S; Wolosker, H; Snyder, SH
The Journal of Biological Chemistry null
Aichurok Kamalova et al.
Cell reports, 32(3), 107921-107921 (2020-07-23)
The anterior thalamus (AT) is critical for memory formation, processing navigational information, and seizure initiation. However, the molecular mechanisms that regulate synaptic function of AT neurons remain largely unexplored. We report that AMPA receptor auxiliary subunit GSG1L controls short-term plasticity
Xue-Yan He et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), E5645-E5654 (2016-09-08)
AMPA-type glutamate receptors (AMPARs) mediate fast excitatory neurotransmission and predominantly assemble as heterotetramers in the brain. Recently, the crystal structures of homotetrameric GluA2 demonstrated that AMPARs are assembled with two pairs of conformationally distinct subunits, in a dimer of dimers
Ahmad H Alhowail et al.
Heliyon, 7(7), e07456-e07456 (2021-07-24)
Doxorubicin (Dox) is a chemotherapeutic agent used widely to treat a variety of malignant cancers. However, Dox chemotherapy is associated with several adverse effects, including "chemobrain," the observation that cancer patients exhibit through learning and memory difficulties extending even beyond
Balakrishnan Selvakumar et al.
Neuropharmacology, 77, 28-38 (2013-09-17)
Behavioral sensitization to cocaine is associated with increased AMPA receptor (AMPAR) surface expression in the nucleus accumbens (NAc). This upregulation is withdrawal-dependent, as it is not detected on withdrawal day (WD) 1, but is observed on WD7-21. Its underlying mechanisms
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