AB2267

Anti-LCN2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): lipocalin 2, migration-stimulating factor inhibitor, siderocalin, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, lipocalin 2 (oncogene 24p3), neutrophil gelatinase-associated lipocalin, Lipocalin-2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005555
• UniProt-Hinterlegungsnummer: P80188
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LCN2(3934)

Beschreibung

Allgemeine Beschreibung

LCN2 (also known as Neurophil Gelatinase-Associated Lipocalin or NGAL) belongs to a superfamily of lipocalins, a collection of small, diversely functional, extracellular proteins. NGAL is secreted from neutrophil granules in three different forms; a monomer of 25 kDa, a disulfide-linked homodimer at 46 kDa, and a disulfide-linked heterodimer with MMP-9 at 135-kDa. It is widely known to induce development of kidney epithelia, to bind and traffic iron, and to act as a molecular signal under inflammatory conditions, such as Crohn’s Disease. Multiple carcinoma studies have also shown elevated expression levels of LCN2 in several different human tumors affecting such organs as: colon, lung, breast, ovaries and pancreas. Additionally, there has been great interest in this protein as a possible marker for: onset of Acute Kidney Injury following cardiac surgery, the progression of Chronic Kidney Disease (CKD), and the survival chances of patients with Chronic Heart Failure (CHF).

Spezifität

This antibody recognizes LCN2.

Immunogen

Epitope: Mature chain

KLH-conjugated linear peptide corresponding to the mature chain of human LCN2.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurodegenerative Krankheiten

Anti-LCN2 Antibody detects level of LCN2 & has been published & validated for use in WB, IH.

Immunohistochemistry Analysis: A 1:200 dilution of Anti-LCN2 antibody from a previous lot detected LCN2 in human spleen tissue.

Qualität

Evaluated by Western Blot in human kidney tissue lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected LCN2 in 10 µg of human kidney tissue lysate.

Zielbeschreibung

LCN2 band observed at 25 kDa Heavy concentration of tissue lysate and a 5 minute exposure time were used to generate the band of interest.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Human kidney tissue lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable