AB125
Anti-Serotonin Antibody
serum, Chemicon®
Synonym(e):
5-HT
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Speziesreaktivität
rat
Hersteller/Markenname
Chemicon®
Methode(n)
immunohistochemistry: suitable
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Spezifität
Serotonin (5-Hydroxytryptamine)
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
Immunogen
5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
Anwendung
Research Category
Neurowissenschaft
Neurowissenschaft
Research Sub Category
Neurotransmitter & Rezeptoren
Neurotransmitter & Rezeptoren
Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Physikalische Form
Rabbit antiserum. Liquid with 0.05% sodium azide.
Lagerung und Haltbarkeit
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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