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Merck

524625

Millipore

Phosphatase Inhibitor Cocktail II

liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.

Synonym(e):

Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.77
Form:
liquid
Lagertemp.:
−20°C
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.

Qualitätsniveau

Form

liquid

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze

Versandbedingung

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.

Anwendung

Phosphatase Inhibitor Cocktail Set II has been used:
  • in triple detergent buffer to lyse cultured cells to extract total protein for western blots[1]
  • as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins[2]
  • as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2) [2]
  • as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting[3]

Biochem./physiol. Wirkung

Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.

Leistungsmerkmale und Vorteile

  • Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
  • Economical: ready-to-use format minimizes wastage.
  • Effective: one ml is adequate for approximately 100 ml extraction buffer.
  • Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).

Komponenten

Provided as a set of five 1 ml vials containing the following components:
  • Imidazole (200 mM)
  • Sodium Fluoride (100 mM)
  • Sodium Molybdate (115 mM)
  • Sodium Orthovanadate (100 mM)
  • Sodium Tartrate Dihydrate (400 mM)      

Warnhinweis

Toxicity: Standard Handling (A)

Sonstige Hinweise

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Piktogramme

Health hazardExclamation mark

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Lagerklassenschlüssel

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)

CAS No.

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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