5.08605

Sodium Orthovanadate, Activated

• Synonym(e): Sodium Orthovanadate, Activated, Orthovanadate, Activated, Vanadate, Activated
• Empirische Formel (Hill-System): Na₃O₄V
• Molekulargewicht: 183.91
• UNSPSC-Code: 12352202
• NACRES: NB.21

Eigenschaften

• Form: liquid
• Qualitätsniveau: 100
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Farbe: colorless
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.

Biochem./physiol. Wirkung

Primary Target
Phosphatases

Reversible: yes

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Toxic (F)

Physikalische Form

Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Sonstige Hinweise

Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany