2520-M
Blot Restore Membrane Rejuvination Kit, 10x
About This Item
Empfohlene Produkte
Hersteller/Markenname
Chemicon®
Methode(n)
western blot: suitable
Nachweisverfahren
chemiluminescent
Allgemeine Beschreibung
It is widely believed that antigen-containing membranes used for Western blot experiments should never be dried. However, this is not true. The Blot Restore kit allows multiple re-use of membranes that were developed with substrate and subsequently dried. In model experiments both PVDF and nitrocellulose membranes showed excellent performance after having been dried for at least two months.
Kit contains two components. Solutioin A re-hydrates dry membranes and washes out chemiluminescent developing reagents. Solution B strips both primary and secondary (labeled) antibodies off the membrane. Treated membranes are ready for blocking and subsequent re-probing with antibodies of choice.
Use of the Blot Restore Kit offers several advantages:
1) Conservation of samples that are expensive or available only in limited quantities,
2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,
3) Re-analysis of anomalous results and confirmation with the same or a different antibody,
4) Correcting errors in incubation with the wrong antibody,
5) Cost savings in reagents and time by reusing the same blot.
Anwendung
The Blot Restore Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.
This product is for research use only; not for diagnostic or in vivo use.
Komponenten
Blot Restore Solution B (10x) - (1 container, 50 mL).
Lagerung und Haltbarkeit
Rechtliche Hinweise
Haftungsausschluss
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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