251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• Synonym(e): DAF-2, 4,5-Diaminofluorescein
• Empirische Formel (Hill-System): C₂₀H₁₄N₂O₅
• CAS-Nummer: 205391-01-1
• Molekulargewicht: 362.34
• MDL-Nummer: MFCD05865100
• UNSPSC-Code: 12171500
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥98% (HPLC)
• Form: solution
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: pale yellow
• Fluoreszenz: λ_ex ~495 nm; λ_em ~515 nm
• Lagertemp.: −20°C
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• InChIKey: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Biochem./physiol. Wirkung

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Warnhinweis

Toxicity: Irritant (B)

Physikalische Form

A 5 mM (1 mg in 550 µl) solution in DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Rechtliche Hinweise

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flammpunkt (°C): 87 °C - closed cup - (Dimethylsulfoxide)