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Merck

1.50481

Supelco

SeQuant ZIC®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 150 mm x 0.3 mm capillay Column

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About This Item

UNSPSC-Code:
41115709

product name

SeQuant ZIC-HILIC (5µm,200Å) 150 x 0.3 mm, suitable for HPLC

Materialien

stainless steel column

Qualitätsniveau

Agentur

suitable for USP L114

Produktlinie

SeQuant

Leistungsmerkmale

endcapped: no

Parameter

400 bar pressure
70 °C max. temp.

Methode(n)

HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)

Säule L × ID

15 cm × 0.3 mm

Matrix

fully porous particle

Aktive Matrixgruppe

ZIC-HILIC bonding phase

Partikelgröße

5 μm

Porengröße

200 Å pore size

Betriebs-pH-Wert

2-7.5

Trenntechnik

hydrophilic interaction (HILIC)

Lagertemp.

no temp limit

Allgemeine Beschreibung

SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 150 mm length x 0.3 mm ID is the optimal choice for the Capillary Chromatography separation of complex samples with high sensitivity.
Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances. SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Rechtliche Hinweise

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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