1.50446

SeQuant^® ZIC^®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

• UNSPSC Code: 41115709
• NACRES: NB.21

Eigenschaften

• Produktname: SeQuant^® ZIC-HILIC^™ 3.5µm,200Å 50 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 500 bar pressure; 70 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 5 cm × 4.6 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC bonding phase
• particle size: 3.5 μm
• pore size: 200 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit
• Quality Level: 100

Beschreibung

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

General description

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the separation of polar compounds using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany