14-773-M

BTK (E41K) Protein, active, 10 µg

• UNSPSC-Code: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100; 300
• Mol-Gew.: M_w 78.4 kDa
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• NCBI-Hinterlegungsnummer: NM_000061
• UniProt-Hinterlegungsnummer: P35991 (mouse); Q06187

Beschreibung

Allgemeine Beschreibung

Expressed by baculovirus in Sf21 insect cells

N-terminal His6-tagged, recombinant, full-length, human BTK

Specific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).

Anwendung

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

Research Category
Inflammation & Immunology

Biochem./physiol. Wirkung

• Protein Target: BTK
• Target Sub-Family:TK

Verpackung

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-773M when ordering the 250 μg size.

Qualität

Routinely evaluated by phosphorylation of LKBtide.

Physikalische Form

Format: Ni²⁺/NTA-agarose

Lagerung und Haltbarkeit

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediat

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable