14-772-M

ULK2, active

• UNSPSC-Code: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Mol-Gew.: M_w 62 kDa
• Hersteller/Markenname: Upstate^®
• Methode(n): activity assay: suitable (kinase)
• NCBI-Hinterlegungsnummer: NM_014683
• UniProt-Hinterlegungsnummer: Q8IYT83

Beschreibung

Allgemeine Beschreibung

N-terminal GST and C-terminal 6His-tagged, recombinant, human, ULK2, amino acids 1–306. Expressed by baculovirus in Sf21 insect cells

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of
mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may
govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons

Verpackung

Also available in
10μg
size as catalog #
14-772
and in
250 μg
as catalog #
14-772M
.

Qualität

Routinely evaluated by phosphorylation of casein

Physikalische Form

Format: Purified using glutathione agarose.

Protein in 50mM Tris/HCl pH7.2, 300mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.

Lagerung und Haltbarkeit

Store at -70°C

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Gefahreneinstufungen: Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable