1.13726

HPTLC plates, Silica gel 60 RP-2 F₂₅₄s

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support

• Synonym(e): Reverse Phase Silica Gel 60 Plates
• UNSPSC-Code: 41115711
• NACRES: NB.21

Eigenschaften

• Materialien: glass support; silica gel 60 matrix
• Qualitätsniveau: 100
• Leistungsmerkmale: binder Organic Polymer; fluorescent indicator
• Verpackung: pkg of 25 plates
• Methode(n): thin layer chromatography (TLC): suitable
• Schichtdicke: 200 μm
• Platte L × B: 10 cm × 10 cm
• Partikelgröße: 5-6 μm
• Porengröße: 60 Å medium pore diameter
• Lagertemp.: 2-30°C

Beschreibung

Allgemeine Beschreibung

25 Glass plates 10 x 10 cm

RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Verlinkung

Replaces: 13726-5; 13726

Hinweis zur Analyse

Spezifische Oberfläche (nach BET; 5-Pkt. Messung): 480 - 540 m²/g
Porenvolumen (N₂-Isotherme): 0.74 - 0.84 ml/g
d 50 (Laserbeugung, Kornanalyse): 5 - 7 µm
Schichtdicke: 150 - 200 µm
Dickenschwankung: ≤ 35 µm
Anwendungstechnische Prüfungen:
A) Cholesterintest
hRf-Werte
- Cholesterin, Cholesterintest:
77 - 89
B) Steroidtest
- Methyltestosteron, Steroidtest: 57 - 77
- Reichstein S, Steroidtest: 66 - 86
- Hydrocortison,Steroidtest: 70 - 90
Typischer Wert gemessen an einer aktivierten Platte (120°C, 15 Min)
Eluent a) Aceton/ Wasser (95/5 v/v)
Eluent b) Acetonitril/ Wasser (70/30 v/v)