07-722

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody

Upstate^®, from rabbit

• Synonym(e): Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, kinase insert domain receptor (a type III receptor tyrosine kinase), vascular endothelial growth factor receptor 2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: zebrafish, human
• Speziesreaktivität (Voraussage durch Homologie): primate (based on 100% sequence homology), horse (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), Danio (based on 100% sequence homology)
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NM_002253.2
• UniProt-Hinterlegungsnummer: P35968
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (pTyr1054)
• Angaben zum Gen: human ... KDR(3791)

Beschreibung

Allgemeine Beschreibung

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.

Spezifität

Recognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1054 (human).

Immunogen

Synthetic peptide encompassing

Anwendung

Research Category
Zelluläre Signaltransduktion

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB.

Qualität

Western Blot Analysis:
This lot detected VEGFR2 phosphorylated on Tyr1054 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).

Zielbeschreibung

152 kDa

Physikalische Form

Double affinity purified

Affinity purified rabbit polyclonal IgG in PBS with 0.05% NaN3 and 50% glycerol.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
CSF-1 treated PAE cell lysate, transfected with CSF/KDR.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2