06-659

Anti-phospho-c-Jun (Ser73) Antibody

Upstate^®, from rabbit

• Synonym(e): Activator protein 1, Jun activation domain binding protein, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, enhancer-binding protein AP1, jun oncogene, v-jun sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, human
• Speziesreaktivität (Voraussage durch Homologie): Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• Hersteller/Markenname: Upstate^®
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_002219.1
• UniProt-Hinterlegungsnummer: P05412
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer73)
• Angaben zum Gen: human ... JUN(3725) ; mouse ... Jun(16476) ; rat ... Jun(24516) ; zebrafish ... Jun(335916)

Beschreibung

Allgemeine Beschreibung

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced

signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Spezifität

This antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).

Immunogen

KLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.

Anwendung

Anti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.

Western Blot Analysis :
A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

Qualität

Evaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.

Zielbeschreibung

~40 kDa

Verlinkung

Replaces: 04-213

Physikalische Form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Hinweis zur Analyse

Control
Untreated and anisomycin-treated NIH/3T3 cell lysate.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1