06-597

Anti-phospho-Histone H1 Antibody

Upstate^®, from rabbit

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: bovine, human
• Verpackung: antibody small pack of 25 μg
• Hersteller/Markenname: Upstate^®
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_005319
• UniProt-Hinterlegungsnummer: P16403
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (not specified)
• Angaben zum Gen: human ... HIST1H1C(3006)

Beschreibung

Allgemeine Beschreibung

Histone H1 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H1 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

The N-terminal tail of histone H1 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spezifität

Recognizes phospho-Histone H1, Mr 32 kDa. Additional proteins were also detected, Mr 66 and 100 kDa.

Immunogen

Hyper-phosphorylated isoform of tetrahymena Histone H1 purified by cation-exchange HPLC

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Anti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB.

Qualität

Immunoblot Analysis: 0.5-2 μg/mL detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells (Catalog # 17-306).

Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol/5% glacial acetic acid (v/v).

Zielbeschreibung

32 kDa

Physikalische Form

Protein A purified

Format: Purified

Immunoaffinity Purified immunoglobulin in 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA containing no preservatives.

Lagerung und Haltbarkeit

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.